Space crop production systems are being developed to grow fresh produce in-situ to supplement the astronauts’ diet, but the required ventilation rates for crops in different gravity environments remains poorly understood. The technical papers were not presented in person due to the inability to hold the event as scheduled in Lisbon, Portugal because of the COVID-19 global pandemic. The proceedings for the 2020 International Conference on Environmental Systems were published from July 31 – August 6, 2020. ICES500: Life Science/Life Support Research Technologies Wheeler, National Aeronautics and Space Administration (NASA), Kennedy Space Center, Florida, 32899, US Massa, National Aeronautics and Space Administration (NASA), Kennedy Space Center, Florida, 32899, US Lawrence Koss, National Aeronautics and Space Administration (NASA) Kennedy Space Center, Amentum Services, Inc., Florida, 32899, USA Michael Gildersleeve, National Aeronautics and Space Administration (NASA) Internships and Fellowships (NIF)/Out in Science, Technology, Engineering, and Mathematics (oSTEM) Internship Program, Kennedy Space Center, Florida, 32899, US and Cornell University, New York, 13112, US To reflect viable cells found in the samples, RNA was also detectable using a modified, single-step reverse transcription reaction.Lucie Poulet, National Aeronautics and Space Administration (NASA) Postdoctoral Program, Universities Space Research Association (USRA), Kennedy Space Center, Florida, 32899, US Total heterotrophic counts could also be detected using a 16S gene marker that can identify up to 98% of all bacteria. Escherichia coli, Salmonella enterica Typhimurium, and Pseudomonas aeruginosa were detected at low levels using real-time DNA amplification. The RAZOR EX, a ruggedized, commercial off the shelf, real-time PCR field instrument was tested for its ability to detect microorganisms at low concentrations within one hour. Therefore, a more expedient, low-cost, in-flight method of microbial detection, identification, and enumeration is warranted. To identify and enumerate ISS microbes requires that samples be returned to Earth for complete analysis. The culture based method detects approximately 1-10% of the total organisms present and provides no identification. This increases the microbial load while detecting only a limited number of the total microorganisms. Current microbial characterization methods require enrichment of microorganisms and at least a 48-hour incubation time. ECLSS, environmental control and life support systems). Microorganisms introduced to the ISS are readily transferred between crew and subsystems (i.e. The likelihood of introducing new microorganisms occurs with every exchange of crew or addition of equipment or supplies. Previous research has shown that potentially destructive microorganisms and human pathogens have been detected on the International Space Station (ISS). The 45th International Conference on Environmental Systems was held in Bellevue, Washington, USA on 12 July 2015 through 16 July 2015. Hummerick, Vencore, Inc., Kennedy Space Center, USAĭavid J. Birmele, Sierra Lobo, Inc, Kennedy Space Center, USA Khodadad, Sierra Lobo, Inc, Kennedy Space Center, USA
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